polyclonal rabbit anti mtnr1a  (Bioss)

Journal: The Journal of Neuroscience

Article Title: Selective Enhancement of REM Sleep in Male Rats through Activation of Melatonin MT₁Receptors Located in the Locus Ceruleus Norepinephrine Neurons

doi: 10.1523/jneurosci.0914-23.2024

Figure Lengend Snippet: Figure 3. Immunohistochemical expression of the MT1 receptor in norepinephrine neurons of the LC. A, Schematical representation of the LC taken for the Western blot analysis. B, Immunoblotting with anti-MT1 (1:200) and anti–α-tubulin (aT; 1:10,000) antibodies. Upper bands correspond to the positive blotting from the endogenous control α-tubulin protein whereas the lower bands correspond to the positive blotting from the MT1 protein. MT1 receptors decorated bands of approximately 40 kDa, at their expected molecular weight. The right panel displays the immunoblot with the immunogen peptide. Each lane represents the blotting from one rat. C, Immunoreactivity of MT1 receptors in the LC, 5× (top) and 20× (bottom) show both sides of the LC nucleus expressing MT1 receptors. Scale bar: 250 µm (top), 100 µm (bottom). D, Double immunofluorescence confirmed the expression of MT1 receptors (red) in LC-NE neurons (TH, green). Sections were counterstained with DAPI (blue). Scale bar: 100 µm, 20 µm (expand). E, Percentage of immunoreactivity of neuronal colocalization (yellow), MT1 cellular expression alone (red), and TH neuronal expression alone (green).

Article Snippet: The membranes were incubated (3 h) in a blocking buffer containing 5% nonfat milk in 0.1 M PBS and then rabbit polyclonal anti-MT1 antibodies (AMR-031, conc 1:1,000 in 5% nonfat milk in PBS-Tween 20 0.2% buffer, Alomone Labs) overnight at 4°C.

Techniques: Immunohistochemical staining, Expressing, Western Blot, Control, Molecular Weight

Journal: Animals : an open access journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes.

doi: 10.3390/ani15010083

Figure Lengend Snippet: Figure 3. Distribution of MT1 melatonin receptors in camel spermatozoa, evaluated by the indirect immunofluorescence method. Immunostaining in the head (H), post-acrosome (PA), neck (N), tail (T) and cytoplasmic droplet (CD) was evidenced. Magnification 1000×. MT1 receptors (A,D,G,J), Hoechst staining (B,E,H,K), and bright field (C,F,I,L) are shown.

Article Snippet: Following three additional PBS washes, the spermatozoa were incubated with the primary antibody for melatonin receptor MT1 (MTNR1A mouse polyclonal antibody; Abnova, Taipei, Taiwan; Cat# H00004543-A01, Animals 2025, 15, 83 5 of 17 RRID: AB_462681) diluted 1:10 in PBS with 1% BSA, or the primary antibody for melatonin receptor MT2 (MTNR1B Rabbit Polyclonal Antibody, Acris Antibodies GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198) diluted 1:20 in PBS with 1% BSA overnight at 4 ◦C in a wet chamber.

Techniques: Immunofluorescence, Immunostaining, Staining

Journal: Animals : an open access journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes.

doi: 10.3390/ani15010083

Figure Lengend Snippet: Figure 5. Indirect immunofluorescence controls. Samples were incubated with only the MT1 (A) or MT2 (C) primary or secondary antibody (E) and (G) for Alexa Fluor 594 anti-mouse antibody and Alexa Fluor 488 anti-rabbit antibody, respectively). Magnification 1000×. Fluorescence after 30 s exposition (A,C,E,G) and bright field (B,D,F,H) are shown.

Article Snippet: Following three additional PBS washes, the spermatozoa were incubated with the primary antibody for melatonin receptor MT1 (MTNR1A mouse polyclonal antibody; Abnova, Taipei, Taiwan; Cat# H00004543-A01, Animals 2025, 15, 83 5 of 17 RRID: AB_462681) diluted 1:10 in PBS with 1% BSA, or the primary antibody for melatonin receptor MT2 (MTNR1B Rabbit Polyclonal Antibody, Acris Antibodies GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198) diluted 1:20 in PBS with 1% BSA overnight at 4 ◦C in a wet chamber.

Techniques: Immunofluorescence, Incubation, Fluorescence

Journal: Animals : an open access journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes.

doi: 10.3390/ani15010083

Figure Lengend Snippet: Figure 6. Comparison among age classes of males at different melatonin receptors localizations (MT1 (A) and MT2 (B)) in acrosome (A), post-acrosome (PA), head (H), neck (N), tail (T), cytoplasmic droplet (CD) and apical edge (AE). Values are shown as percentage of localization of n = 439 spermatozoa. * indicates p < 0.05.

Article Snippet: Following three additional PBS washes, the spermatozoa were incubated with the primary antibody for melatonin receptor MT1 (MTNR1A mouse polyclonal antibody; Abnova, Taipei, Taiwan; Cat# H00004543-A01, Animals 2025, 15, 83 5 of 17 RRID: AB_462681) diluted 1:10 in PBS with 1% BSA, or the primary antibody for melatonin receptor MT2 (MTNR1B Rabbit Polyclonal Antibody, Acris Antibodies GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198) diluted 1:20 in PBS with 1% BSA overnight at 4 ◦C in a wet chamber.

Techniques: Comparison